Procedure for the genetic identification of european species of the maja genus (crabs). A protocol has been developed for the genetic identification of european atlantic spider crab (maja brachydactyla) against the other species of the genus found in the mediterranean (m. Squinado, m. Crispata and m. Goltziana). These species constitute a commercial resource of great value; its identification is fundamental to enable its traceability and proper labeling and to guide its management and conservation. The protocol consists of amplifying a 671 bp DNA region of the 16s gene of mitochondrial DNA, whose nucleotide sequence is characteristic of each of the 4 species. In particular, 3 restriction enzymes have been identified that digest the 671 bp DNA fragment, so that the combination of the band patterns they generate is distinctive and characteristic of each of the species.
This invention offers the advantage of being able to be applied to any individual regardless of its vital phase, which is essential for the development of recovery and repopulation programs for each species. It also highlights that it is a simple and fast method and the equipment needed for molecular analysis is basic.
Applications
The main applications of the invention are identification of species for traceability and correct labeling in the market and the identification of species for the development of recovery and repopulation programs, ensuring the correct incorporation of each species in its geographical area of distribution.
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