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University of Southampton are leading developers of Raman scattering based and other label-free detection and imaging technologies .For label-free non-culture based direct detection they have developed a novel spectroscopic methodology that can identify and differentiate bacterial biofilms at the genus, species and the strain level, as desired.
Their Raman capability also allows the quantification of composition and purity of treatments or therapeutics, without and within biofilms
Devices that are portable, robust and that can provide point-of-care diagnosis (for triage) or in-line analysis are under development. These have a unique capability for rapid, multimodal, label-free imaging that can provide chemical and structural composition of biofilms including matrix and microbes. These could be immensely useful for clinical and industrial applications to understand, visualise or monitor the effect (and changes pre- and post-application) of drugs or treatments.
As an example, their imaging capability can allow the visualisation of penetration of drugs into biofilms or other biological matrices (such as in-vitro models, skin) non-invasively, non-destructively and in real-time, if so desired. They are already taking these technologies nearer to the patient in an NIHR-funded AMR (anti-microbial resistance) laboratory at the University Hospital Southampton. This patient-line-of-sight facility will enable clinical feasibility testing of their easy and simple diagnostic test(s) on patient biofluid samples and enable studies on therapeutic benefit.
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National Biofilms Innovation Centre, where we connect and collaborate with academics and industrial partners to harness the UKs academic and industrial strengths in Biofilms for economic and Societal benefit. we are funded by BBSRC and Innovate UK and the Hartree centre.
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