Chemo-enzymatic covalent deposition of functional and reporter groups at 3’-terminal nucleotides in small RNAs such as miRNAs, siRNAs or long ssRNAs using HEN1 RNA 2’˗O-methyltransferases from plants or animals, respectively. Enables a highly selective analysis of transcriptomes and discovery of new microRNA species in animal and human samples.
PURPOSE
Targeted covalent labeling of small RNAs orssRNAs for imaging, enrichment and sequence analysis.
FIELDS OF APPLICATION
Biomedicine, biomarkers and diagnostics, miRNA profiling, transcriptome profiling, single-molecule genomics, biomaterials.
INTELLECTUAL PROPERTY
Patent applications: EP3271478 (A1) and US20180251814 (A1).
Applicant: Vilnius University.
PUBLICATIONS
Plotnikova et al. (2014) J. Am. Chem. Soc., 136: 13550.
Osipenko et al. (2017)Angew. Chem.Int.Ed., 56: 6507.
Mickutė et al. (2018)Nucleic Acids Res., 46: e104.
Tomkuvienė et al. (2019)Curr.Opin.Biotechnol., 55: 114.
Current development status
Laboratory prototypes
Vilnius University is the largest Lithuanian higher education and research institution. We are open for collaborative research, contract research or any cooperation activities with partners from business and public organizations. Multidisciplinary approach on R&D activities at Vilnius University provides unique opportunity to incorporate methodologies, perspectives, data and concepts from all scientific fields – biomedical, physical sciences, technologies, social sciences and humanities. We believe that the right mix of shared intentions with our partners and clients, outstanding teams of university researchers and open access to modern R&D infrastructure deliver remarkable solutions that can change organizations, markets and society in general.
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