Researchers of the University of Granada have developed probes based on nanoparticles, both fluorescent and functionalized with metals, which act as dual probes, and can be used for both flow cytometry (FACS –Fluorescent-Activated Cell Sorting) and mass cytometry (CyTOF -Cytometry by Time-Of-Flight-).
The probes can be used for bar coding of living cells or carrying out in vivo studies of different cell lineages through cell co-cultures as well as the activation of drug precursors through cytoplasmic catalysis of organometallic reactions inside living cells.
The invention depicts the next aspects:
1. Dual Probes
The probes comprise nanoparticles both fluorescent and functionalized with metals which are suitable for carrying out both flow cytometry (FACS) and mass cytometry (CyTOF).
The nanoparticles are functionalized with a fluorophore so they are converted into fluorescent probes, as well as with metallic isotopes (e.g. palladium). The specific combination of a Fluorophore and specific metallic isotope confer a dual feature, allowing its detection through flow cytometry (fluorophore) and mass cytometry (metallic isotope).
These nanoparticles enter mammalian cells without a previous permeabilization step, without showing cytotoxic effects. These unique features open the option to do studies over living cells.
2. Cell codification procedure
The invention allows the cell codification through barcoding.
The living cells, through a nanofection process (introducing nanoparticles in cells), incorporate the metallic-fluorescent probes inside, which have a unique and predefined combination. Due to that specific combination, which is detected through mass cytometry and flow cytometry, the probes can act as bar codes.
In that way, a simultaneous reading of different cell lines can be made through cell co-cultures, since each cell line will have a specific and unique combination of isotopes and fluoride.
3. Synthesis procedure
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The mission of the Research Results Transfer Office (OTRI, for its initials in Spanish) of the University of Granada (UGR) is to identify and valorize R&D results for their commercialization by industry and entities worldwide.
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