Several prior-art techniques such as ion mobility spectrometry and gas-phase HDX measurements have been used for assessing the conformational properties of gaseous protein ions. Most of these approaches comprise the use of ion traps or ICR cells where a gas is introduced and the ions are incubated with the gas. However, such approaches are associated with potential limitations such as extended gas-phase labeling time, complex exchange kinetics, and comparatively low gas pressure. This novel approach enables a rapid gas-phase isotopic labeling of protein ions for enhanced detection of protein conformations.
Value Proposition
The approach:
•Involves use of a curtain labeling technique wherein the protein ions are allowed to pass through a cloud of deuterated gas for labeling
•Allows for an effective control over gas-phase labeling time along with obtaining higher operational gas pressure as compared to conventional techniques
•Offers unique advantages and flexibility for analytical measurements over prior-art approaches
•Would be commercially useful for determination of protein stoichiometry along with architecturing of large functional protein assemblies
Intellectual Property status
Pending Utility Application 13/264,574
Pending Canada National Application 2,758,917
Pending European National Application 10765098.8
License status
Available for license
Direct Link:http://neu.technologypublisher.com/technology/20052