The “Ronit 1 Library” is a human synthetic single-chain Fv antibody library of 2?109 independent clones where human complementarity-determining region (CDR) gene fragments of any germ line have been incorporated combinatorially into the appropriate positions of variable-region general frameworks. VH-DP47, VL-DPL3 and VL-DPK22 were used as master frameworks for variable domains of the heavy, lambda light and kappa light chains, respectively. The antibody genes in the library were built on single master frameworks into which diverse CDRs were allowed to recombine. These CDRs were sampled from in vivo-processed gene sequences, thus potentially optimizing the levels of correctly folded and functional molecules, and resulting in a molecule exhibiting a lower immunogenicity compared to naive immunoglobulins.
The repertoire of scFvs represented in the “Ronit 1 library” is cloned into pCC16 phagemid vector. As such, the scFvs may be displayed on phage by infection of the host E. coli with a helper phage or may be expressed as soluble scFvs.
Project ID : 2-2012-348
The Technology
The “Ronit 1 Library” is a human synthetic single-chain Fv antibody library of 2´109 independent clones where human complementarity-determining region (CDR) gene fragments of any germ line have been incorporated combinatorially into the appropriate positions of variable-region general frameworks. VH-DP47, VL-DPL3 and VL-DPK22 were used as master frameworks for variable domains of the heavy, lambda light and kappa light chains, respectively. The antibody genes in the library were built on single master frameworks into which diverse CDRs were allowed to recombine. These CDRs were sampled from in vivo-processed gene sequences, thus potentially optimizing the levels of correctly folded and functional molecules, and resulting in a molecule exhibiting a lower immunogenicity compared to naive immunoglobulins.
The repertoire of scFvs represented in the “Ronit 1 library” is cloned into pCC16 phagemid vector. As such, the scFvs may be displayed on phage by infection of the host E. coli with a helper phage or may be expressed as soluble scFvs.
Potential Applications
A robust tool for in-house antibody discovery
Advantages
Human scFv antibody phage display library· scFvs are fused at their C-termini to the cellulose binding domain (CBD) and can be immobilized onto cellulose either when displayed on phage or when expressed as soluble scFv-CBD fusions
Stage of Development
The “Ronit 1 Library” has been screened against individual peptides and proteins or against protein-peptide complexes and the best selected scFvs exhibited dissociation constants in the subnanomolar range in BIACore analyses.
Reference
JMB (2004) 335, 117-192, "A Human Synthetic Combinatorial Library of Arrayable Single-chain Antibodies based on Shuffling in Vivo Formed CDRs into General Framework Regions
Project manager
Adi Elkeles
BD Manager
Project researchers
Itai Benhar
T.A.U Tel Aviv University, Life Sciences
Molecular Microbiology-Biotechnology
Ramot is Tel Aviv University's (TAU) technology transfer company and its liaison to industry, bringing promising scientific discoveries made at the university to industry's attention. The company provides the legal and commercial frameworks for inventions made by TAU faculty, students and researchers, protecting discoveries with patents and working jointly with industry to bring scientific innovations to the market.
Create your free account to connect with RAMOT at Tel Aviv University Ltd. and thousands of other innovative organizations and professionals worldwide
Send a request for information
to RAMOT at Tel Aviv University Ltd.
Technology Offers on Innoget are directly posted
and managed by its members as well as evaluation of requests for information. Innoget is the trusted open innovation and science network aimed at directly connect industry needs with professionals online.
Need help requesting additional information or have questions regarding this Technology Offer?
Contact Innoget support
