-The FLI I monoclonal antibody is superior to commercial polyclonal antibodies currently available due to its high binding specificity and affinity to detect the EWS::FLI I fusion protein
- Production of renewable clonal hybridoma cells, immunopurified for enhanced specificity and binding affinity
- This antibody can be used successfully in a variety of detection methods to localize EWS::FLI I and study protein-protein interactions and possibly nucleic acid-protein interactions
OVERVIEW
Ewing sarcoma (ES) is a rare tumor that affects children, adolescents, and young adults. This tumor has a high morbidity in all patients and for those who present with metastatic disease a high mortality. A canonical chromosomal translocation, either t(11;22)(q24;p12) or t(21;22)(q22;q12) leads to the fusion oncoproteins EWS::FLI I or EWS::ERG in 95% of ES patients. We recognized a critical need for a stably sourcedcustom FLI I monoclonal antibody that detects with high affinity the target EWS::FLI I fusion protein in Ewings Sarcoma cell lines with a reproducible hybridoma source of antibody.This antibody detects few non-specific bands and is highly suitable for biochemistry experiments including immunoprecipitation, immunohistochemistry, immunoblotting, Western Blots, and ELISA assay techniques and potential other additional applications including chromatin immunoprecipitation and nucleic acid protein interaction studies.This FLI I monoclonal antibody is an improvement over other polyclonal antibodies on the market due to its selective affinity and binding superiority to the fusion protein.
Benefit
high stability
•high affinity
•high specificity
·Hybridoma cell line
Market Application
biochemistry
• research purposes
• detection methods
Monoclonal antibody
Publications
Selvanathan S, Toretsky J. An EWS::FLI I monoclonal antibody with multimodal utility and specificity for FLI I. doi:10.1101/2023.11.13.566886.
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