- Allows indefinite expansion of human germline pluripotent stem cells (gPSCs) from biopsy in a specialized cell culture medium, enabling their use in regenerative medicine.
- Overcomes slow growth, differentiation, graft rejection, and tissue availability issues, facilitating hgPSC transplantation for treating diseases.
- Induces gPSC cell proliferation without additional growth factors and reduces the chance of graft rejection, increasing the cell volume and donor availability.
OVERVIEW
The invention describes method to indefinitely expand human germline pluripotent stem cells (hgPSCs) from biopsy in proprietary cell culture medium. Cells can be transplanted for treating and/or curing diseases.
hgPSCs hold promise for use in regenerative medicine, however these cells grow very slowly making it impractical to utilize gPSCs to realize their potential in regenerative medicine, moreover, because so much time is required to expand these cells in culture, invariably large portions of the cells will begin to differentiate, rendering them unusable for further manipulation. These issues lead to the need for a method of culturing hgPSCs in a continuous manner that can promote rapid expansion, without differentiation.
Georgetown University researchers have developed a hgPSC culturing technique that not only address the issues above, but also overcomes issues of graft rejection and tissue availability. The present invention enables the use of hgPSCs from biopsy to be expanded and transplanted.
BACKGROUND
Germline stem cells, when taken out of their niche, can differentiate into cell types from all three germ layers, making them germline pluripotent stem cells (gPSCs). Spermatogonial stem cells (SSCs) and ovarian stem cells (OSCs) can also transition from unipotent to pluripotent when removed from their natural environment, becoming germline pluripotent stem cells (gPSCs) and germline embryonic stem-like cells (gESLCs). Despite their potential for regenerative medicine, gPSCs have been limited by slow growth and difficulties in expansion without differentiation. Efficient continuous culture methods that enable rapid expansion of gPSCs without differentiation are required to harness their full potential for regenerative medicine applications.
Benefit
This technique induces gPSC cell proliferation without requiring additional exogenous growth factors and J2 feeder system.
Uses cultured cells from biopsy reducing chances of graft rejection in transplant recipients.
Increases hgPSC volume and alleviates scarcity of donors.
Absence of animal components improves transplantation safety.
Market Application
Publications
US Patent No.16/759,352
Re-Defining Stem Cell-Cardiomyocyte Interactions: Focusing on the Paracrine Effector Approach.J Stem Cells Regen Med.2018; 14(1): 10–26.Published online 2018 May 30.doi:10.46582/jsrm.1401003
Our mission is to advance GU’s innovations through strategic alliances and new venture creation, to facilitate the translation of research breakthroughs into tangible solutions, and to cultivate a dynamic and inclusive environment for entrepreneurship. We advance this mission in support of the GU community and for the benefit of society.
Create your free account to connect with Georgetown University and thousands of other innovative organizations and professionals worldwide
Send a request for information
to Georgetown University
Technology Offers on Innoget are directly posted
and managed by its members as well as evaluation of requests for information. Innoget is the trusted open innovation and science network aimed at directly connect industry needs with professionals online.
Need help requesting additional information or have questions regarding this Technology Offer?
Contact Innoget support
