Seeking Analytical Platform to Characterize Biofilm

For our industry, irreversible attachment of microorganisms to surface (e.g., fabrics, gum, toilet, etc.) and biofilm architecture are among the key factors of biofilm formation. We need a holistic analytical platform to characterize the chemical composition (substances like proteins (e.g., fibrin), polysaccharide (e.g., alginate), quorum sensing molecules, as well as eDNA) of biofilms and the architecture of the biofilm. The platform will be integrated with microbiology to elucidate MOA and identify the targets to intervene biofilm formation.

WHAT WE ARE LOOKING FOR

• One stop shop for analytical characterization of biofilms
• In situ chemical characterization (cryo OrbiSIMS) with chemical filtering method
• Chemicals to monitor: polysaccharides, lipids, glycolipids, protein fragments or peptides, quorum sensing molecules, metabolites
• 3D Chemistry: spatial resolution of the biofilm
• Visualization of biofilm: Cryo TEM, hyperspectral imaging, etc.

WHAT WE ARE NOT LOOKING FOR

Isolated capabilities for analytical characterization of biofilm